recombinant mouse il 4 r d systems  (R&D Systems)

Journal: International Journal of Inflammation

Article Title: Resident Corneal Cells Communicate with Neutrophils Leading to the Production of IP-10 during the Primary Inflammatory Response to HSV-1 Infection

doi: 10.1155/2012/810359

Figure Lengend Snippet: Cultured fibroblast cells produce IP-10 in response to stimulation with proinflammatory mediators. 4 × 10 4 cultured mouse corneal fibroblast cells were incubated at 37°C and 5% CO 2 in 500 μ L serum-free media with or without cytokine stimulation for 24 h. Cells were stimulated with 10 ng/mL of either recombinant mouse IL- α or IFN- γ and assayed for IP-10 production by ELISA.

Article Snippet: Recombinant mouse IL-1 α and IFN- γ ) were purchased from R&D Systems (Minneapolis, MN.).

Techniques: Cell Culture, Incubation, Recombinant, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Inflammation

Article Title: Resident Corneal Cells Communicate with Neutrophils Leading to the Production of IP-10 during the Primary Inflammatory Response to HSV-1 Infection

doi: 10.1155/2012/810359

Figure Lengend Snippet: Purified mouse neutrophils produce high levels of IP-10 in response to stimulation with proinflammatory mediators or HSV-1. 1 × 10 6 neutrophils purified from bone marrow were stimulated for 8 h in vitro with 10 ng/mL of either recombinant mouse IL-1 α , IFN- γ , or HSV-1 at the dose indicated. Chemokine production was assayed by ELISA. An asterisk indicates a value not significantly different from the unstimulated control.

Article Snippet: Recombinant mouse IL-1 α and IFN- γ ) were purchased from R&D Systems (Minneapolis, MN.).

Techniques: Purification, In Vitro, Recombinant, Enzyme-linked Immunosorbent Assay